Neurocircuit Control of Digestion and Metabolism   Using CRISPR/Cas9 mouse models and genetically targeted viral vectors, we are matching neuron populations with their roles controlling the function of the stomach, pancreas, and other visceral organs.   Pictured above: sensory (red) and motor (green) parasympathetic innervation of the muscular layer of the mouse stomach.

Neurocircuit Control of Digestion and Metabolism

Using CRISPR/Cas9 mouse models and genetically targeted viral vectors, we are matching neuron populations with their roles controlling the function of the stomach, pancreas, and other visceral organs.

Pictured above: sensory (red) and motor (green) parasympathetic innervation of the muscular layer of the mouse stomach.

  Molecular Taxonomy of Neural Cell Types   Through high-throughput single-cell transcriptomics (Drop-seq, sNuc-Seq),  we are systematically defining cell types that comprise and connect key brain regions in energy balance.    Pictured above: clustering analysis of ~21,000 single-cell transcriptomes from in and around the mouse arcuate hypothalamus (Campbell  et al ., 2017)

Molecular Taxonomy of Neural Cell Types

Through high-throughput single-cell transcriptomics (Drop-seq, sNuc-Seq),  we are systematically defining cell types that comprise and connect key brain regions in energy balance. 

Pictured above: clustering analysis of ~21,000 single-cell transcriptomes from in and around the mouse arcuate hypothalamus (Campbell et al., 2017)

  Roles for Non-Neuronal Cells in Energy Balance   Our unbiased single-cell transcriptomics analyses have suggested novel roles in energy balance for non-neuronal cell types, including the tanycytes stained in yellow (Vimentin) and red (Sprr1a) above.   Pictured above: immunofluorescence of Vimentin (yellow), Sprr1a (red), Cck::GFP (green), and DNA (blue) at the arcuate and median eminence border.

Roles for Non-Neuronal Cells in Energy Balance

Our unbiased single-cell transcriptomics analyses have suggested novel roles in energy balance for non-neuronal cell types, including the tanycytes stained in yellow (Vimentin) and red (Sprr1a) above.

Pictured above: immunofluorescence of Vimentin (yellow), Sprr1a (red), Cck::GFP (green), and DNA (blue) at the arcuate and median eminence border.